High-resolution polarization-sensitive Fourier ptychography microscopy using a high numerical aperture dome illuminator
Journal article, Peer reviewed
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Original versionGholami Mayani, M., Tekseth, K. R., Breiby, D. W., Klein, J. & Akram, M. N. (2022). High-resolution polarization-sensitive Fourier ptychography microscopy using a high numerical aperture dome illuminator. Optics Express, 30(22), 39891-39903. https://doi.org/10.1364/OE.469115
Polarization-sensitive Fourier-ptychography microscopy (pFPM) allows for high resolution imaging while maintaining a large field of view, and without mechanical movements of optical-setup components. In contrast to ordinary light microscopes, pFPM provides quantitative absorption and phase information, for complex and birefringent specimens, with high resolution across a wide field of view. Using a semi-spherical home-built LED illumination array, a single polarizer, and a 10x /0.28NA objective, we experimentally demonstrate high performance pFPM with a synthesized NA of 1.1. Applying the standard quantitative method, a measured half-pitch resolution of 244 nm is achieved for the 1951 USAF resolution test target. As application examples, the polarimetric properties of a herbaceous flowering plant and the metastatic carcinoma of human liver cells are analyzed and quantitatively imaged.