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dc.contributor.authorWilson, Abbey E.
dc.contributor.authorSergiel, Agnieszka
dc.contributor.authorSelva, Nuria
dc.contributor.authorSwenson, Jon
dc.contributor.authorZedrosser, Andreas
dc.contributor.authorStenhouse, Gordon
dc.contributor.authorJanz, David M.
dc.date.accessioned2022-03-21T10:51:39Z
dc.date.available2022-03-21T10:51:39Z
dc.date.created2021-04-07T12:55:07Z
dc.date.issued2021
dc.identifier.citationWilson, A. E., Sergiel, A., Selva, N., Swenson, J. E., Zedrosser, A., Stenhouse, G. & Janz, D. M. (2021). Correcting for enzyme immunoassay changes in long term monitoring studies. MethodsX, 8, Artikkel 101212.en_US
dc.identifier.issn2215-0161
dc.identifier.urihttps://hdl.handle.net/11250/2986409
dc.description.abstractEnzyme immunoassays (EIAs) are a common tool for measuring steroid hormones in wildlife due to their low cost, commercial availability, and rapid results. Testing technologies improve continuously, sometimes requiring changes in protocols or crucial assay components. Antibody replacement between EIA kits can cause differences in EIA sensitivity, which can hinder monitoring hormone concentration over time. The antibody in a common cortisol EIA kit used for long-term monitoring of stress in wildlife was replaced in 2014, causing differences in cross reactivity and standard curve concentrations. Therefore, the objective of this study was to develop a method to standardize results following changes in EIA sensitivity. We validated this method using cortisol concentrations measured in the hair of brown bears (Ursus arctos). • We used a simple linear regression to model the relationship between cortisol concentrations using kit 1 and kit 2. • We found a linear relationship between the two kits (R2 = 0.85) and used the regression equation (kit2 = (0.98 × kit1) + 1.65) to predict cortisol concentrations in re-measured samples. • Mean predicted percent error was 16% and 72% of samples had a predicted percent error <20%, suggesting that this method is well-suited for correcting changes in EIA sensitivity.en_US
dc.language.isoengen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleCorrecting for enzyme immunoassay changes in long term monitoring studiesen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.rights.holder© 2021 The Authors.en_US
dc.source.volume8en_US
dc.source.journalMethodsXen_US
dc.identifier.doihttps://doi.org/10.1016/j.mex.2021.101212
dc.identifier.cristin1902709
dc.source.articlenumber101212en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal