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dc.contributor.authorParulekar, Niranjan Nitin
dc.contributor.authorKolekar, Pandurang
dc.contributor.authorJenkins, Andrew
dc.contributor.authorKleiven, Synne
dc.contributor.authorUtkilen, Hans Christian
dc.contributor.authorJohansen, Anette
dc.contributor.authorSawant, Sangeeta
dc.contributor.authorKulkarni-Kale, Urmila
dc.contributor.authorKale, Mohan
dc.contributor.authorSæbø, Mona
dc.date.accessioned2018-06-05T08:44:32Z
dc.date.available2018-06-05T08:44:32Z
dc.date.created2017-12-30T16:38:44Z
dc.date.issued2017
dc.identifier.citationPLoS ONE. 2017, 12 (3), 1-22.nb_NO
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11250/2500272
dc.description.abstractInteractions between different phytoplankton taxa and heterotrophic bacterial communities within aquatic environments can differentially support growth of various heterotrophic bacterial species. In this study, phytoplankton diversity was studied using traditional microscopic techniques and the bacterial communities associated with phytoplankton bloom were studied using High Throughput Sequencing (HTS) analysis of 16S rRNA gene amplicons from the V1-V3 and V3-V4 hypervariable regions. Samples were collected from Lake Akersvannet, a eutrophic lake in South Norway, during the growth season from June to August 2013. Microscopic examination revealed that the phytoplankton community was mostly represented by Cyanobacteria and the dinoflagellate Ceratium hirundinella. The HTS results revealed that Proteobacteria (Alpha, Beta, and Gamma), Bacteriodetes, Cyanobacteria, Actinobacteria and Verrucomicrobia dominated the bacterial community, with varying relative abundances throughout the sampling season. Species level identification of Cyanobacteria showed a mixed population of Aphanizomenon flos-aquae, Microcystis aeruginosa and Woronichinia naegeliana. A significant proportion of the microbial community was composed of unclassified taxa which might represent locally adapted freshwater bacterial groups. comparison of cyanobacterial species composition from HTS and microscopy revealed quantitative discrepancies, indicating a need for cross validation of results. To our knowledge, this is the first study that uses HTS methods for studying the bacterial community associated with phytoplankton blooms in a Norwegian lake. The study demonstrates the value of considering results from multiple methods when studying bacterial communities.nb_NO
dc.language.isoengnb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleCharacterization of bacterial community associated with phytoplankton bloom in a eutrophic lake in South Norway using 16S rRNA gene amplicon sequence analysisnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.rights.holder2017, Parulekar et al.nb_NO
dc.source.pagenumber1-22nb_NO
dc.source.volume12nb_NO
dc.source.journalPLoS ONEnb_NO
dc.source.issue3nb_NO
dc.identifier.doi10.1371/journal.pone.0173408
dc.identifier.cristin1533050
cristin.unitcode222,58,1,0
cristin.unitnameInstitutt for natur, helse og miljø
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal