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dc.contributor.authorUtheim, Øygunn Aass
dc.contributor.authorPasovic, Lara
dc.contributor.authorRæder, Sten
dc.contributor.authorEidet, Jon Roger
dc.contributor.authorFostad, Ida
dc.contributor.authorSehic, Amer
dc.contributor.authorRoald, Borghild
dc.contributor.authorde la Paz, Maria Fideliz
dc.contributor.authorLyberg, Torstein
dc.contributor.authorDartt, Darlene A.
dc.contributor.authorUtheim, Tor Paaske
dc.date.accessioned2019-10-25T08:22:37Z
dc.date.available2019-10-25T08:22:37Z
dc.date.created2019-06-07T14:58:20Z
dc.date.issued2019
dc.identifier.citationPLoS ONE. 2019, 14 (3).nb_NO
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11250/2624362
dc.descriptionThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.nb_NO
dc.description.abstractPurpose Transplantation of limbal stem cells is a promising therapy for limbal stem cell deficiency. Limbal cells can be harvested from either a healthy part of the patient’s eye or the eye of a donor. Small explants are less likely to inflict injury to the donor site. We investigated the effects of limbal explant size on multiple characteristics known to be important for transplant function. Methods Human limbal epithelial cells were expanded from large versus small explants (3 versus 1 mm of the corneal circumference) for 3 weeks and characterized by light microscopy, immunohistochemistry, and transmission electron microscopy. Epithelial thickness, stratification, outgrowth, ultrastructure and phenotype were assessed. Results Epithelial thickness and stratification were similar between the groups. Outgrowth size correlated positively with explant size (r = 0.37; P = 0.01), whereas fold growth correlated negatively with explant size (r = –0.55; P < 0.0001). Percentage of cells expressing the limbal epithelial cell marker K19 was higher in cells derived from large explants (99.1±1.2%) compared to cells derived from small explants (93.2±13.6%, P = 0.024). The percentage of cells expressing ABCG2, integrin β1, p63, and p63α that are markers suggestive of an immature phenotype; Keratin 3, Connexin 43, and E-Cadherin that are markers of differentiation; and Ki67 and PCNA that indicate cell proliferation were equal in both groups. Desmosome and hemidesmosome densities were equal between the groups. Conclusion For donor- and culture conditions used in the present study, large explants are preferable to small in terms of outgrowth area. As regards limbal epithelial cell thickness, stratification, mechanical strength, and the attainment of a predominantly immature phenotype, both large and small explants are sufficient.nb_NO
dc.language.isoengnb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleEffects of explant size on epithelial outgrowth, thickness, stratification, ultrastructure and phenotype of cultured limbal epithelial cellsnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.rights.holder© 2019 Utheim et al.nb_NO
dc.source.pagenumber22nb_NO
dc.source.volume14nb_NO
dc.source.journalPLoS ONEnb_NO
dc.source.issue3nb_NO
dc.identifier.doi10.1371/journal.pone.0212524
dc.identifier.cristin1703532
dc.relation.projectHelse Sør-Øst RHF: 2010092nb_NO
cristin.unitcode222,56,2,0
cristin.unitnameInstitutt for optometri, radiografi og lysdesign
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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