| dc.description.abstract | Plastic waste is a significant threat to wildlife and marine life, presenting a potential for a biotechnological based on biodegradation using bacteria that break down polymers in plastic into monomers or oligomers, allowing them to be disposed of more quickly (Khan and Majeed 2019). This work aims to isolate and grow microorganisms that can degrade low-density polyethylene (LDPE) and use it as a carbon source. Low-density polyethylene (LDPE) was used as a substrate, which was industrially produced without any additives, and a piece of old plastic was used as a source of microorganisms. Microorganisms were cultured in a growth medium to which (LDPE) was added to obtain only LDPE degradation isolates. The following isolates were obtained (AH1, AH2, AH3, AH4, AH5, AH1a, AH1b, AH3b1, AH4b1, AH4b2, AH4b3, AH3a, AH4a, and AH1a). Each of the isolates (AH1, AH2, AH3, AH4, AH5, AH1a, AH1b, AH4b3, AH4b2) was marked by staining and some tests were performed to characterize them. The isolates were identified by 16s RNA is sequencing. Some analytical tests such as the growth test, the CO2 produced test, and a weight loss test was also performed to monitor biodeterioration. Only AH2 showed growth in the first stage of growth measurement of isolates(AH1, AH2, AH3, AH4, and AH5), and only AH4b2 reported growth in the second stage of growth measurement of isolates (AH4b2, AH4b3, AH1a, and AH1b). The isolates (AH4b2 and AH2) both showed negative growth during the growth confirmation stage. When measuring the amount of carbon dioxide produced and calculating the weight loss for the isolates (AH4b2 and AH2), negative results were obtained, which indicates the unreliability of biodegradation and it requires more investigation. | |
