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dc.contributor.authorEika, Elisabeth
dc.date.accessioned2015-03-04T12:52:10Z
dc.date.accessioned2017-04-19T13:13:35Z
dc.date.available2015-03-04T12:52:10Z
dc.date.available2017-04-19T13:13:35Z
dc.date.issued2014
dc.identifier.citationEika, E. Mecillinam resistance in clinical isolates of Escherichia coli. Master thesis, Telemark University College, 2014
dc.identifier.urihttp://hdl.handle.net/11250/2438901
dc.description.abstractResistance to the β-lactam mecillinam was observed in 11 clinical isolates of Escherichia coli (MCisolates) from urinary tract infections. How this resistance arose, is not known. The clinical isolates differ from normal E. coli by showing abnormal cell shape and slow growth. Formation of the normal E. coli rod shape involves two gene clusters, mrd and mre located respectively at 14 min and 71 min on the E. coli chromosomal map, mrd/mre mutants may show mecillinam resistance. This study attempt to test whether the MC isolates are mrd/mre mutants by introducing plasmids carrying mrd/mre by electroporation and looking for restoration of normal morphology and mecillinam sensitivity. Attempts to introduce a plasmid carrying the mrd genes were only successful for one of the eleven MC isolates. The plasmid did not restore the normal phenotype – i.e. the strain kept its mecillinam resistance and coccoid morphology. Because of the lack of results, different approaches to introduce desired genes have been theoretically explored
dc.language.isoeng
dc.publisherHøgskolen i Telemark
dc.subjectEscherichia coli
dc.subjectE. coli
dc.titleMecillinam resistance in clinical isolates of Escherichia coli
dc.typeMaster thesis
dc.description.versionPublished version
dc.rights.holder© Copyright The Author. All rights reserved
dc.subject.nsi472


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