Point of Care COPD Diagnostics Based on Paper-based Assay and Biofluid Sensing
Doctoral thesis
Published version
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https://hdl.handle.net/11250/3071019Utgivelsesdato
2023Metadata
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Sammendrag
Chronic obstructive pulmonary disease (COPD) has always been the one of the top four major
death causes during the last ten years. During the era of covid-19 pandemic, COPD patients
faced higher mortality rates than ever before. Inefficiency in COPD diagnosis and exacerbation
monitoring leads to tremendous medical costs and economic burden. Decentralized point-ofcare
(PoC) diagnostics based on disposable paper-based biosensors emerge as a cost-effective
approach, which requires sensitive detection of COPD biomarkers in human saliva and blood
as a prerequisite. When testing biological samples, it also needs to consider matrix effects
resulted from samples and handling protocols. Biofouling is the most noticeable and
deleterious effect of untreated saliva or serum that happens simultaneously on most
interfaces that results in blocking and passivation of the sensing surface with non-specific
adsorbed proteins.
This dissertation work focuses on the following challenges: (i) demonstrate sensitive
electrochemical and optical biosensing of COPD biomarkers in human saliva based on lateral
flow assays; (ii) investigate solutions to resolve hook effect in sandwich LFAs and redesign the
LFA structure; (iii) control defects in emerging nanomaterials, taken 2-dimensional black
phosphorus as an example, for the development of future biosensors that contact human
saliva and blood; and (iv) an anti-biofouling strategy to overcome non-specific protein
adsorption and matrix effect of body fluids.
Består av
Article 1: He, G., Dong, T., Yang, Z., Branstad, A., Huang, L., Jiang, Z., (2022). Point-of-care COPD diagnostics: Biomarkers, sampling, paper-based analytical devices, and perspectives, Analyst, 147, 1273-1293. Doi: 10.1039/D1AN01702K (Not included online)Article 2: He, G., Dong, T., Yang, Z., Jiang, Z., (2022) Mitigating hook effect in one-step quantitative sandwich lateral flow assay by timed conjugate release, Talanta, 240, 123157. Doi: 10.1016/j.talanta.2021.123157
Article 3: He, G., Dong, T., Yang, Z., Ohlckers, P., (2019) Tuning 2D Black Phosphorus: defect tailoring and surface functionalization, Chemistry of Materials, 31(24), 9917-9938. Doi: 10.1021/acs.chemmater.9b03639 (Not included online)
Article 4: He, G., Dong, T., et al. (2023) Surface oxygen deficiency enabled spontaneous anti-proteinfouling in WO3 nanosheets. (Not included online)
Article 5: (IEEE Conference) He, G., Dong, T., Yang, Z. (2022) Probe sonication to prepare homogenous WO3 nanosheet inks for energy conversion and biosensing applications, 20th International Conference on Mechatronics-Mechatronika, pp. 1-4. IEEE. DOI: 10.1109/ME54704.2022.9982863. (Not included online)