Effects of fertilizer and glyphosate on soil microbial diversity and pathogens in two soil types in Telemark

Abstract

The occurrence of microorganisms in the soil depends on numerous physical and chemical factors. Microorganisms play a central role in the environment and plant growth. New molecular techniques are currently being used for metagenomic studies which reveal species composition and data output in the form of DNA sequences. This study is an attempt to understand soil microbiome, its analysis and to see the effects of fertilizer and pesticide on rhizospheres’ bacteria. We performed an experiment to study the effects of treatment on microbial diversity for a time span of four months. The combination of 16S rRNA amplification and next generation sequencing provided information on microbes and their diversity. Further analysis examines the effects of fertilizer and pesticide on microbial diversity. Functional analysis reveals the effects of various soil treatments on potential pathogens in mixed and sandy soil samples. The sequencing output demonstrates to discussion on advantages and drawbacks of the sequencing method and sheds light on physical properties of the soil.

Keywords: Microbial diversity, rhizosphere, pathogens, metagenomics, glyphosate

The occurrence of microorganisms in the soil depends on numerous physical and chemical factors. Microorganisms play a central role in the environment and plant growth. New molecular techniques are currently being used for metagenomic studies which reveal species composition and data output in the form of DNA sequences. This study is an attempt to understand soil microbiome, its analysis and to see the effects of fertilizer and pesticide on rhizospheres’ bacteria. We performed an experiment to study the effects of treatment on microbial diversity for a time span of four months. The combination of 16S rRNA amplification and next generation sequencing provided information on microbes and their diversity. Further analysis examines the effects of fertilizer and pesticide on microbial diversity. Functional analysis reveals the effects of various soil treatments on potential pathogens in mixed and sandy soil samples. The sequencing output demonstrates to discussion on advantages and drawbacks of the sequencing method and sheds light on physical properties of the soil.

Keywords: Microbial diversity, rhizosphere, pathogens, metagenomics, glyphosate