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dc.contributor.authorJenkins, Andrew
dc.contributor.authorRaasok, Cecilie
dc.contributor.authorPedersen, Benedikte Nevjen
dc.contributor.authorJensen, Kristine
dc.contributor.authorAndreassen, Åshild Kristine
dc.contributor.authorSoleng, Arnulf
dc.contributor.authorEdgar, Kristin Skarsfjord
dc.contributor.authorLindstedt, Heidi Elisabeth H.
dc.contributor.authorKjelland, Vivian
dc.contributor.authorStuen, Snorre
dc.contributor.authorHvidsten, Dag
dc.contributor.authorKristiansen, Bjørn Erik
dc.date.accessioned2019-11-26T12:28:13Z
dc.date.available2019-11-26T12:28:13Z
dc.date.created2019-09-02T10:24:05Z
dc.date.issued2019
dc.identifier.citationBMC Microbiology. 2019, 19.nb_NO
dc.identifier.issn1471-2180
dc.identifier.urihttp://hdl.handle.net/11250/2630524
dc.descriptionOpen Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.nb_NO
dc.description.abstractBackground Candidatus Neoehrlichia mikurensis is an emerging tick-borne pathogen. It is widely distributed in Ixodes ricinus ticks in Europe, but knowledge of its distribution in Norway, where I. ricinus reaches its northern limit, is limited. In this study we have developed a real time PCR test for Ca. N. mikurensis and used it to investigate the distribution of Ca. N. mikurensis in Norway. Results Real time PCR targeting the groEL gene was developed and shown to be highly sensitive. It was used to detect Ca. N. mikurensis in 1651 I. ricinus nymphs and adults collected from twelve locations in Norway, from the eastern Oslo Fjord in the south to near the Arctic Circle in the north. The overall prevalence was 6.5% and varied locally between 0 and 16%. Prevalence in adults and nymphs was similar, suggesting that ticks acquire Ca. N. mikurensis predominantly during their first blood meal. In addition, 123 larvae were investigated; Ca. N. mikurensis was not found in larvae, suggesting that transovarial transmission is rare or absent. Sequence analysis suggests that a single variant dominates in Norway. Conclusions Ca. N. mikurensis is widespread and common in ticks in Norway and reaches up to their northern limit near the Arctic Circle. Ticks appear to acquire Ca. N. mikurensis during their first blood meal. No evidence for transovarial transmission was found.nb_NO
dc.language.isoengnb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleDetection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL genenb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.rights.holder© The Author(s). 2019nb_NO
dc.source.pagenumber12nb_NO
dc.source.volume19nb_NO
dc.source.journalBMC Microbiologynb_NO
dc.identifier.doi10.1186/s12866-019-1502-y
dc.identifier.cristin1720475
cristin.unitcode222,58,1,0
cristin.unitcode222,58,3,0
cristin.unitnameInstitutt for natur, helse og miljø
cristin.unitnameInstitutt for prosess-, energi- og miljøteknologi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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